Ref. CDVS003
192 tests/kit
SWINE INFLUENZA
SWINE
Swine Influenza
Swine Influenza is an enzyme immunoassay (EIA) for the detection of specific IgG antibodies against Swine Influenza virus antigen in pigs (and rabbits) sera of vaccinated or convalescent animals of grip.
General Information
At the beginning of the XX century in the United States the Swine
Influenza appeared regularly. In Europe, the first isolations of the virus
took place in Italy in 1976, due to a clinical process whose symptoms were
very similar to the ones described in the United States. Also in Belgium
in 1979, in France in 1980 and in Spain in 1981 isolations have been
described, so the spreading of the disease is clear.
It is mainly detected in feeding places. Some cases have been also
detected in gestant sows with some subsequent abortive problem.
The most clear symptoms of the disease are the simultaneous appearance of
respiratory symptoms, high fever (40-41°C) and anorexia.
It spreads quickly in the exploitations, in just 3 or 4 days, and if there
are not further bacterial complications, the pigs can recover in 8 days.
In an influenza outbreak there are great economic losses, not so much for
the mortality which generally does not go beyond 1%, but rather by the
delay in the animals growth.
Laboratory diagnosis is carried out in a direct form, with isolation of
the influenza virus in chichen’s embryos, stemming from nasal hyssops
and in an indirect form, using serology through the demonstration of a
seroconversion or a high presence of IgG in animal sera which have not
been vaccinated.
The ELISA technique of influenza allows us to know very quickly and
clearly the possible presence of antibodies against the influenza A virus,
which will provide data to diagnose and control respiratory processes in
feeding places, value vaccination programs etc.
Principle of the test
The Cypress Diagnostic Swine Influenza test is based on the principle
of an enzyme Immunoassay (EIA). The assay system utilizes an inactivated
specific antigen. This specific antigen is coated on the microtiter well.
First, the test sample or controls are allowed to react with the solid
phase specific antigen. If there are specific antibodies, they will bound
to the antigen. After 20 minutes incubation at 37°C, the wells are washed
with the washing solution to remove unbound material.
In a second step, an protein A/HRPO conjugate is added to the wells
resulting in the specific antibody against Swine Influenza being
sandwiched between the solid phase antigen and enzyme conjugate protein-A.
After 20 minutes incubation at 37°C, the wells are washed with the
washing solution to remove unbound material.
A substrate/chromogen solution is added and incubated 10 minutes ,
resulting in the development of a blue color in the wells where a complex
Antigen/ Anti-Influenza antibody/Protein-A : HRPO conjugate was formed
previously.
The color development is stopped with the addition of the stop reaction
solution and the color turns yellow. The yellow color is measured
spectrophotometrically at 450 nm.
The concentration of pig specific IgG antibody against Swine Influenza is
proportional to the color intensity of the test sample.
Rev.09.95
