Ref.CDVS004
192 tests/kit
SR/MR
SWINE
SE/MR
SR/MR is an enzyme immunoassay(EIA) for the detection of specific IgG antibodies against Erysipelothrix rhusiopathiae bacterium antigens in pigs sera of vaccinated or convalescent animals of the swine erysipelas disease.
General Information
Swine Erysipelas is a disease caused by Erysipelothrix rhusiopathiae
bacterium.
Swine Erysipelas is a widespread disease and causes serious economic loss.
Although it occurs in pigs of all ages, those of 3 months to 1 year are
most susceptible, and the acute form of the disease in unweaned pigs has
become increasingly common.
The incidence of infection is variable and may be particularly high in
some areas.
The disease may manifest in several forms : acute and fatal infection,
mild infection (urticarial form), and chronic form. The latter includes
both the cardiac and arthritic types. It also is important to take the
abortive form, very frequent in our exploitations, into consideration.
Spread of infection among animals occurs from ingestion of organisms
present in the faeces, urine or vomit of clinically diseased animals, and
in the faeces and urine of symptomless carriers. Infection is rarely
disseminated by contamination of skin wounds.
Stained smears are used for diagnosis in acute cases, but it isn’t
sensitive for detection of chronic infections.
The Enzymeimmunoassay (EIA) is a very sensitive and specific tool for the
diagnosis of every form of infection, chronic and symptomless included. It
is at the same time, very useful in epidemiological studies, determination
of animal farm state of health, and vaccination control.
Principle of the test
The Cypress Diagnostic SE/MR test is based on the principle of an
enzyme Immunoassay (EIA). The assay system utilizes an inactivated
specific antigen. This specific antigen is coated on the microtiter well.
First, the test sample or controls are allowed to react with the solid phase specific antigen. If there are specific antibodies, they will bound to the antigen. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material.
In a second step, a Protein A/HRPO conjugate is added to the wells resulting in the specific antibody against Erysipelothrix rhusiopathiae being sandwiched between the solid phase antigen and enzyme conjugate Protein A. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material. A substrate/chromogen solution is added and incubated 20 minutes , resulting in the development of a blue color in the wells where a complex Antigen/Anti-Erysipelothrix antibody/Protein A - HRPO conjugate was formed previously.
The color development is stopped with the addition of the stop reaction
solution and the color turns yellow. The yellow color is measured
spectrophotometrically at 450 nm.
The concentration of pig specific IgG antibody against Erysipelothrix rhusiopathiae is proportional to the color intensity of the test sample.
Rev.09.95
