TRT
Poultry

TRT

TRT is an enzyme immunoassay(EIA) for the detection of specific IgG antibodies against Turkey Rhinotracheitis (TRT) virus antigens in poultry sera of vaccinated or convalescent animals of the disease.

General Information

TRT virus is a Pneumovirus which causes an infection in the high respiratory tract of turkeys. The first cases appeared in the 70’s in South Africa.
The finding in England of antibodies against Turkey Rhinotracheitis(TRT) virus in poultry herds, which had suffered from the Swell Heat Syndrome (SHS) opened the possibility of search, on a wide scale, for the implication of this virus in SHS.
In Spain as in France, serological evaluations performed in poultry herds affected by SHS, showed a clear seroconversion to TRT virus.
Thus, it is confirmed that this virus is intensely implicate in the SHS process in chicken, fowl and turkeys.
At present, SHS may be found in most countries with industrial poultry farming. In each place where
SHS is present, the major technical concern has been the search of implicate infectious agents.
So, it has showed condition of high respiratory tract with tracheal ciliostasis but its pathogenicity is highly important with the collaboration of secondary agents as E. coli, Pasteurella multocida and haemolitica, Haemophilus sp, etc...
The disease causes an important respiratory complex in affected poultry lots, with variable mortality rates and their economic repercussions are very important.
It has been showed by mean of assays of the kit evolution, that in particular cases, as for detection of antibodies produced by alive vaccinal antigens, it might be necessary the use of other complementary techniques, as IFI for greater evaluation of results.

Principle of the test

The Cypress Diagnostic TRT test is based on the principle of an enzyme Immunoassay (EIA). The assay system utilizes an inactivated specific antigen. This specific antigen is coated on the microtiter well.
First, the test sample or controls are allowed to react with the solid phase specific antigen. If there are specific antibodies, they will bound to the antigen. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material.
In a second step, an Anti-Chicken IgG/HRPO conjugate is added to the wells resulting in the specific antibody against Turkey Rhinotracheitis virus being sandwiched between the solid phase antigen and enzyme conjugate antibody. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material.
A substrate/chromogen solution is added and incubated 15 minutes , resulting in the development of a blue color in the wells where a complex Antigen/ Anti-TRT virus antibody/Anti-Chicken IgG-HRPO conjugate was formed previously.
The color development is stopped with the addition of the stop reaction solution and the color turns yellow. The yellow color is measured spectrophotometrically at 450 nm.
The concentration of poultry specific IgG antibody against Turkey Rhinotracheitis (TRT) virus is proportional to the color intensity of the test sample.

Rev.09.95

© 1999 HLS Veterinary Biological Products, Inc.