Ref.CDVO008
192 tests/kit
MYCOPLASMA AGALACTIAE
Ovine
Mycoplasma Aglactiae
Mycoplasma Agalactiae is an enzyme immunoassay(EIA) for the detection of specific IgG antibodies against Mycoplasma Agalactiae antigens in sheep sera of vaccinated or convalescent animals of the contagious agalaxia disease.
General Information
Contagious Agalaxia is a disease produced in the little ruminants by
different species or the Mycoplasma genus. In the bovines etiology is
reduced to Mycoplasma agalactiae, while in the goats intervene other
species of Mycoplasma such as M. Mycoides, M. capricolum, etc...
Contagious Agalaxia is an acute disease spread especially in the countries
of the Mediterranean Basin an it is greatly relevant in those countries in
which little ruminants are exploited for the production of milk.
In the contagious Agalaxia, the infection by Mycoplasma is in most cases
produced orally and in an intramammary way, its incubation period
oscillating between 12 and 48 hours.
The disease produced by Mycoplasma Agalactiae in Spain implies the 100% of
the forms of contagious ovine Agalaxia ant the 90% of the goats. This
infection can manifest itself in an acute, subacute, chronic or
non-apparent form.
An inadequate anamnesis along with a significant sampling, will provide
the laboratory with the appropriate tools for the detection of the casual
agent of a contagious Agalaxia process.
Bacteriological diagnosis of the different Mycoplasma sp. is possible in
reasonable periods of time (approximately 1 week).
The serological diagnoses (ELISA) have shown a better performance in
regard to the previous techniques used (fixing of the complement).
Principle of the test
The Cypress Diagnostic Mycoplasma Agalactiae test is based on the
principle of an enzyme Immunoassay (EIA). The assay system utilizes an
inactivated specific antigen. This specific antigen is coated on the
microtiter well.
First, the test sample or controls are allowed to react with the solid
phase specific antigen. If there are specific antibodies, they will bound
to the antigen. After 20 minutes incubation at 37°C, the wells are washed
with the washing solution to remove unbound material.
In a second step, an Anti-Sheep IgG/HRPO conjugate is added to the wells
resulting in the specific antibody against Mycoplasma agalactiae being
sandwiched between the solid phase antigen and enzyme conjugate antibody.
After 20 minutes incubation at 37°C, the wells are washed with the
washing solution to remove unbound material.
A substrate/chromogen solution is added and incubated 10 minutes ,
resulting in the development of a blue color in the wells where a complex
Antigen/ Anti-Mycoplasma agalactiae antibody/Anti-Sheep IgG-HRPO conjugate
was formed previously.
The color development is stopped with the addition of the stop reaction
solution and the color turns yellow. The yellow color is measured
spectrophotometrically at 450 nm.
The concentration of sheep specific IgG antibody against Mycoplasma
agalactiae is proportional to the color intensity of the test sample.
Rev.09.95
