Ref.CDVF031
48 tests/kit
SCV - PEROX
Spring Viraemia of Car - Fish
SCV - PEROX
Immunoenzyme assay for the detection of spring viraemia of carp (SVC) virus in cell cultures.
General Information
Spring viraemia of carp is a contagious viral disease of the Cyprinidae.
Other species, such as the sheatfish(Silurus glanis) are also sensitive to
this virus (Pasco et al., 1987). The cause of the disease is a rhabdovirus
named Rhabdovirus carpio (Fijan et al., 1971) which has been described in
detail by de Kinkelin and Le Berre (1974). This viral infection is found
in all parts or Europe where Cyprinidae are farmed, but the disease also
exist in other areas. It affects fish of all ages, the most typical
victims being the "one - summer" try at the time of the spring
warming of the water (Baudouy et al., 1980). The disease carries with it a
high mortality rate. The clinical signs of contamination are petechial
hemorrhages of the skin and gills, dark coloring of the tegument,
exophthalmia and a distended abdomen. Loss of balance is also seen in
diseased fish. The internal lesions are characterized by petechial
hemorrhages of the viscera, fibrinous peritonitis, and catarrhal or
necrotic enteritis. While the serological traces of a Rhabodovirus carpio
infection indicate that serology may be a valid alternative for studying
the health status of a carp population (Hattenberger - Baudouy et al.
1989), laboratory diagnosis of the disease usually involves identification
of the virus in cell cultures.
Principle of the test
The infected specimens are ground up in a mortar with the help of sand, then put in solution in an antibiotic - supplemented culture medium. The preparation is centrifuged and a 24- well cell culture plate inoculated with a serial dilution of the supernatant.
After 1 hour incubation at optimal temperature culture medium is added to each well and the plate is incubated until a cytopathogenic effect is observed. At this point, the cell preparation is fixed, then rinsed. SVC - specific monoclonal antibody is then added and the plate returned to the incubator. After this first incubation with monoclonal antibody the plate is rinsed. then the conjugate, goat anti-mouse peroxidase (peroxidase - coupled - mouse - immunoglobulin - specific goat immunoglobulin) is added to each well and the plate incubated once more. The plat is then rinsed and the chromogen/substrate mixture added to each well. The cell layer is then observed under an inverted microscope. If the virus causing spring viraemia of carp is present a red color will appear after a few minutes at the sites of viral replication.
Rev.11.95
