Ref.CDVB006
192 tests/kit
IBR
Bovine
IBR
IBR is an enzyme immunoassay(EIA) for the detection of specific IgG antibodies against Infectious bovine rhinotracheitis (IBR) virus antigens in cattle sera.
General Information
Infectious bovine rhinotracheitis (IBR) is an infectious disease caused
by an Herpes-virus and it’s characterized by conditions basically at the
respiratory and genital or reproductive level.
It’s a very common disease, as it has been showed in a large amount of
serological studies performed in several countries. Serological tests are
indicating a progressive increase of the disease.
IBR virus causes an infectious disease in young and adult animals.
The three best known forms are :
-Respiratory : The mode of transmission is basically by aerosol.
Incubation period is variable, depending on the cases, and it may reach 2
months. Therefore the relationship with other factors which lead to it is
high. General symptoms consist of anorexia basically, strong hyperthermia
(40 to 42°C), hypersalivation, seromucous excretions, coughing,
conjunctivitis. All these may last up to 8 days. The most characteristic
lesions are fibrinous and haemorrhagical tracheitis, bronchitis, and
necrothics lesions in pharynx and larynx.
-Genital : It spreads by coitus. Its incubation period is from 24 to 48
hours, and it’s characterized by generalization of pustules in vagina
and vulva.
-Abortive : It’s another expression of IBR virus, since without prior
symptoms abortion is produced.
It usually affects the 80% of animals in an exploitation and mortality
rate varies from 2 to 10%, being higher in young animals.
All seropositive not vaccinated animals, are potential expellers of the
virus.
Horizontal transmission of disease is of great importance in bringing
animals of different ages or with different immune states.
Prevention requires to carry out good serological checking in the
exploitations, as well as the application of vaccine plans suitable for
each case.
Principle of the test
The Cypress Diagnostic IBR test is based on the principle of an enzyme
Immunoassay (EIA). The assay system utilizes an inactivated specific
antigen. This specific antigen is coated on the microtiter well.
First, the test sample or controls are allowed to react with the solid phase specific antigen. If there are specific antibodies, they will bound to the antigen. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material.
In a second step, a Protein G/HRPO conjugate is added to the wells resulting in the specific antibody against Infectious bovine rhinotracheitis(IBR) virus being sandwiched between the solid phase antigen and enzyme conjugate Protein G. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material. A substrate/chromogen solution is added and incubated 15 minutes , resulting in the development of a blue color in the wells where a complex specific Antigen/Anti-IBR antibody/Protein G - HRPO conjugate was formed previously.
The color development is stopped with the addition of the stop reaction
solution and the color turns yellow. The yellow color is measured
spectrophotometrically at 450 nm.
The concentration of cow specific IgG antibody against Infectious bovine
rhinotracheitis (IBR) virus is proportional to the color intensity of the
test sample.
Rev.09.95
